Monday, January 31, 2022

Publication: Fish is polairzed - See full optical property of zebrafish

 Our colleague Antonia Lichtenegger recently demonstrated the whole body and full-optical property of a zebrafish. Zebrafish recently attracts the attentions of biological, medical, and pharmaceutical researchers as it can closely mimic human disease and its fast growth enables quick-research cycle.

In this demonstration, wildtype zebrafish at three development stages, namely eight-days, one-month and two-month post fertilization were investigated in vivo using a polarization-sensitive Jones-matrix optical coherence tomography (JM-OCT) prototype. The scatter and polarization characteristics of various zebrafish organs, such as the skin, the muscles, and the eye, were analyzed in three-dimensions, in a label-free and non-destructive fashion. The presented results showed a promising first step for the usability of JM-OCT in zebrafish-based research.

The details of this study can be found in a recently published article in Journal of Biomedical Optics (SPIE).

A. Lichtenegger, P. Mukherjee, J. Tamaoki, L. Bian, L. Zhu, I.G. Abd El-Sadek, S. Makita, K. Leskovar, M. Kobayashi, B. Baumann, Y. Yasuno, "Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography," J. Biomed. Opt. 27, 016001 (2022). https://doi.org/10.1117/1.JBO.27.1.016001

>> Full-length article (open access). 

私達の研究室の Antonia Lichtenegger さんが生きたままのゼブラフィッシュのマルチコントラスト光イメージングについて論文を出版しました。ゼブラフィッシュは成長・進展の早い人体の疾患モデルとして近年生物学、医学、薬学で大きな注目を集める動物です。

 この論文では、我々の開発した Jones matrix OCT (JM-OCT) 顕微鏡を用いて、発生後8日、1ヶ月、2ヶ月という3つのタイミングにおけるゼブラフィッシュの全身、皮膚、筋肉、眼球などの組織・臓器のマルチコントラスト・3次元トモグラフィーイメージングに成功しました。この結果は、今後 JM-OCT がゼブラフィッシュを用いた生物、医学、薬学研究において有用なツールになることを示唆しています。

 この研究の詳細は米光工学会 (SPIE) の Journal of Biomedical Optics に掲載されています。

A. Lichtenegger, P. Mukherjee, J. Tamaoki, L. Bian, L. Zhu, I.G. Abd El-Sadek, S. Makita, K. Leskovar, M. Kobayashi, B. Baumann, Y. Yasuno, "Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography," J. Biomed. Opt. 27, 016001 (2022). https://doi.org/10.1117/1.JBO.27.1.016001

>> Full-length article (open access).